By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Gelder, Grahn, Gregory, Grohmann, GÃ¶tz, Haase, Heuer, Heuer, Heuer, Hill, Hirt, Honda, KlÃ¼mper

Plasmid Transfer and Conjugation Assays. Large plasmids were transferred to clinical isolates via either conjugation or direct electroporation when possible. For transfer by conjugation, the TOP10 strain (Life Technologies, USA) was used as donor strain due to its high competence and leucine auxotrophy.

Creative writing copywright: How to protect work when submitting to potential http://sleighters.com/rugs/pages/index.php?port-fare-series-book-2-release- after separation and (iii) evaluate the market potential for the recovered fractions. to a transglutaminase assay to estimate the extent of the deamidation which will be Designer microbial communities for fermented milk products: A Systems a novel heterogeneous energy-efficient hierarchical architecture and a hybrid Here, we present a novel experimental method to estimate the plasmid mobilization potential of a mixed bacterial community, using IncQ RSF1010 as model plasmid. We quantify the mobilization potential of a model community extracted from a domestic shower conduit. In that work transfer was quantified based 377In conclusion, this method is the first one to assess the plasmid mobilization potential of a microbial 378 community on a quantitative level by estimating its transfer frequency through fluorescent 379 microscopy. Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a Here, we present a novel experimental method to estimate the plasmid mobilization potential of a mixed bacterial community, usingIncQRSF1010asmodelplasmid.Wequantifythemobiliza- tion potential of a model community extracted from a domestic shower conduit.

Novel assay to measure the plasmid mobilizing potential of mixed microbial communities

Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a Here, we present a novel experimental method to estimate the plasmid mobilization potential of a mixed bacterial community, usingIncQRSF1010asmodelplasmid.Wequantifythemobiliza- tion potential of a model community extracted from a domestic shower conduit. Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli KlÃ¼mper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Download PDF (3 MB) Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli KlÃ¼mper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Cite Novel assay to measure the plasmid mobilizing potential of mixed microbial communities . By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Barth Smets , Arnaud Dechesne , and Uli Klümper An individual-based approach to explain plasmid invasion in bacterial populations Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community.

DOI: 10.3389/fmicb.2014.00730 IV Klümper U, Dechesne A, Riber L, Gülay A, Brandt KK, Sørensen SJ, Smets BF. (2015).

2019-10-09

2017-07-12 2014-02-10 Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. By Barth Smets, Arnaud Dechesne, and Uli Klümper. Novel Assay To Assess Permissiveness of a Soil Microbial Community toward Receipt of Mobile Genetic Elements. By Arnaud Dechesne.

Novel assay to measure the plasmid mobilizing potential of mixed microbial communities . By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey,

2016-09-23 · By using plasmid-encoded TEM β-lactamase mediated ampicillin resistances as a proof of principle system, we (1) developed a fluorescence in situ hybridization-test (FISH) capable to detect the respective mRNAs, (2) implemented an immunofluorescence test to identify the corresponding proteins and (3) compared these two microscopic tests with an established colorimetric nitrocefin assay to 2019-08-28 · 39 plasmids, members within complex microbial communities might have a hitherto unrecognized 40 potential to maintain plasmids for long-term community-wide access. 41 available under aCC-BY-NC-ND 4.0 International license. not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F We thank L. Riber and S. J. Sørensen for access to the tagged RSF1010 plasmid, L. K. Jensen for technical assistance in the laboratory and S. M. Milani for assistance in FACS sorting. This work was funded by the Villum Kann Rasmussen Foundation Center of Excellence CREAM (Center for Environmental and Agricultural Microbiology). Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Uli Klümper, Ariadni Droumpali, Arnaud Dechesne, Barth F. Smets.
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Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli Klümper * , Ariadni Droumpali , Arnaud Dechesne and Barth F. Smets * … Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli Klümper*, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets* Department of Environmental Engineering,Technical University of Denmark, Kongens Lyngby, Denmark Edited by: Kornelia Smalla, Julius Kühn-Institut, Germany Reviewed by: Novel assay to measure the plasmid mobilizing potential of mixed microbial communities.
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These range from the very rapid (infectious) spread of traits associated with plasmids like RP4 through a bacterial community, which can eventually occupy up to 100% of potential recipient cells in just a few hours under the appropriate conditions, to the apparent reluctance of structured microbial communities to accept exogenous DNA or active MGEs.

View Article PubMed/NCBI Google Scholar 40 medium supplemented with the antibiotics necessary for plasmid selection (Table S1), harvested by 41 centrifugation and washed in 0.9% NaCl solution. After adjusting cell number (3.0 ×107 cells per 42 ml) by measuring in a Thomas counting chamber, the diluted donor cultures were used in filter 43 mating assays as described previously.5,4 44 Viruses harbor vast potential for diverse genetic content, arrangement, and encoded functions [14,15,16,17]. Recognizing their genetic diversity, there has been substantial interest in “mining” these viral sequences for novel anti-microbial drug candidates, enzymes for biotechnological applications, and for bioremediation [18,19,20,21,22].

av C Atterby · 2019 — In conclusion, wild birds can function as potential resistance reservoirs and sentinels for community carriage of multidrug-resistant bacteria in rural Cambodia is lated with a test bacterium, and then exposed to antibiotics have been report- ed. types (SP48, SP165 and novel ST) had functioned as plasmid recipients in.

149 bacterial communities toward conjugal plasmids by quantification and isolation of Smets, B. F. Novel assay to measure the plasmid 156 mobilizing potential of mixed microbial communities. Front. Microbiol. 2014, 5 (December), Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli KlÃ¼mper, Ariadni Droumpali, Arnaud Dechesne & Barth F. Smets; Frontiers in Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli eKlümper, Ariadni eDroumpali, Arnaud eDechesne, Barth F Smets; Affiliations Uli eKlümper Technical University of Denmark Ariadni eDroumpali Technical University of Denmark Arnaud eDechesne By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Gelder, Grahn, Gregory, Grohmann, GÃ¶tz, Haase, Heuer, Heuer, Heuer, Hill, Hirt, Honda, KlÃ¼mper 2010-05-28 Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Published in: Frontiers in Microbiology, December 2014 DOI: 10.3389/fmicb.2014.00730: Pubmed ID: 25566238. Authors: Uli KlÃ¼mper, Ariadni Droumpali, Arnaud Dechesne, Barth F. Smets Abstract: Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Uli Klümper; Ariadni Droumpali; Arnaud Dechesne; Barth F. Smets; Frontiers in Microbiology. Published on 21 Dec 2014.

Is Necessary for the Active Form of ParA From Myxococcus pMF1 Plasmid. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Klümper U, Droumpali A, Dechesne A, Smets BF. Front Microbiol, 5:730, 22 Dec 2014 Cited by: 8 articles | PMID: 25566238 | PMCID: PMC4273639. Free to read & use Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Barth Smets , Arnaud Dechesne , and Uli Klümper An individual-based approach to explain plasmid invasion in bacterial populations Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli KlÃ¼mper, Ariadni Droumpali, Arnaud Dechesne & Barth F. Smets; Frontiers in Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Front. Microbiol., 5 (2014), p.